Distribution and some properties of bile salt-binding proteins in rainbow trout, Salmo gairdneri

Trout liver cytosol has proteins that bind bile salts and 8-anilino-1-naphthalene sulphonate and that co-elute from Sephadex G-75. The binding of 8-anilino-1-naphthalene sulphonate is decreased by 90% in the presence of 33 microM lithocholate, implying the proteins are the same. Bile salt-binding protein is present in liver, but was not detected in gills, kidney, muscle or intestine. Bile salt-binding protein, partially-purified by Sephadex G-75 chromatography, was resolved into 2 fractions by cholate-affinity chromatography. Fatty acids do not appear to be bound by bile salt-binding protein. No fatty acid-binding protein was detected in trout liver cytosol.