No effect of smoking on sulfamethazine acetylation

Seven healthy subjects who smoked at least one pack of cigarettes/d were acetylator-phenotyped with sulfamethazine (SMZ) while they were actively smoking, and again at least one month after they had completely stopped. There were no significant differences of the acetylation profiles in the smoking vs. nonsmoking state, as measured by %ASMZ in serum six hours post-SMZ dosing or by %ASMZ in urine five to six hours post-SMZ dosing. We conclude that smoking does not influence SMZ acetylation and probably does not affect drug acetylation in general.