Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Connectin content and its post-mortem changes in fish muscle.

N Seki, T Watanabe

Journal of Biochemistry

|April 1, 1984

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Factors predicting neurosensory disturbance after bilateral sagittal split ramus osteotomy: a retrospective cohort study.

International journal of oral and maxillofacial surgery·2024

Same author

Prebiotic effect of two grams of lactulose in healthy Japanese women: a randomised, double-blind, placebo-controlled crossover trial.

Beneficial microbes·2019

Same author

Stress predictors in two Asian dental schools with an integrated curriculum and traditional curriculum.

European journal of dental education : official journal of the Association for Dental Education in Europe·2018

Same author

Development of leptomeningeal carcinomatosis during a marked response of brain metastases to pembrolizumab in a patient with non-small-cell lung cancer.

Annals of oncology : official journal of the European Society for Medical Oncology·2017

Same author

Cytological markers for predicting ALK-positive pulmonary adenocarcinoma.

Diagnostic cytopathology·2017

Same author

Randomized, double-blind, phase III trial of palonosetron versus granisetron in the triplet regimen for preventing chemotherapy-induced nausea and vomiting after highly emetogenic chemotherapy: TRIPLE study.

Annals of oncology : official journal of the European Society for Medical Oncology·2016

Connectin, a protein in fish and rabbit muscle, changes during aging. Carp muscle shows faster changes in connectin composition compared to rabbit muscle.

Area of Science:

Muscle protein research
Comparative biochemistry

Background:

Connectin is a high molecular weight protein found in myofibrils.
Its role and changes during post-mortem aging are not fully understood across species.

Purpose of the Study:

To compare the content and characteristics of connectin in fish and rabbit skeletal muscles.
To investigate the effects of post-mortem aging on connectin submolecular composition in different species.

Main Methods:

Isolation of connectin from fish and rabbit myofibrils using SDS-gel filtration.
Analysis of connectin composition via SDS-polyacrylamide gel electrophoresis.
Monitoring changes in connectin bands during post-mortem muscle aging.

Main Results:

Related Experiment Videos

Connectin constitutes about 13% of fish myofibrillar proteins and 16% in rabbits, with no significant species variation in fish.
High molecular weight connectins from carp and rabbit exhibit a doublet band (bands 1 and 2), with rabbit band 1 migrating slower than carp band 1.
Post-mortem aging caused band 1 to decrease and band 2 to increase, with further transformation into faster bands, indicating similar aging effects on carp and rabbit connectin.

Conclusions:

Connectin content is similar across fish species and comparable to rabbits.
Post-mortem aging affects connectin structure similarly in fish and mammals.
Carp muscle exhibits a faster rate of connectin band 1 degradation during aging compared to rabbit muscle.