Volatile nitrosamines in the main stream smoke of black tobacco

The cigarettes studied are smoked through an automatic device. The mainstream smoke is trapped in Citrate-Phosphate buffer pH 4.5 containing 20 mM ascorbic acid. The volatile nitrosamines are extracted in dichloromethane, purified on alumina column, then analysed by gas-chromatography with a thermal energy analyzer (TEA) detector. The two main volatile nitrosamines quantified are: Nitrosodimethylamine and Nitrosopyrrolidine.