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Related Experiment Videos

Self-generated electrical currents through Xenopus neurulae.

K R Robinson, R F Stump

    The Journal of Physiology
    |July 1, 1984
    PubMed
    Summary
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    Xenopus neurulae generate an endogenous epithelial current that exits at the blastopore. This sodium-dependent current is similar to that of adult frog skin and is inhibited by amiloride.

    Area of Science:

    • Developmental Biology
    • Electrophysiology
    • Comparative Physiology

    Background:

    • Epithelial current production is crucial for physiological processes.
    • Understanding early developmental currents aids in comprehending tissue differentiation.
    • Xenopus laevis embryos offer a model for studying developmental electrophysiology.

    Purpose of the Study:

    • To investigate endogenous epithelial current production in Xenopus laevis neurulae.
    • To characterize the ionic basis and properties of this embryonic current.
    • To compare the embryonic current to known epithelial transport mechanisms.

    Main Methods:

    • Extracellular vibrating electrode measurements on Xenopus laevis neurulae (stages 14-22) in DeBoer (DB) solution.
    • Ionic substitution experiments (Na+-free solutions, Li+ substitution).

    Related Experiment Videos

  • Pharmacological inhibition using verapamil, ouabain, and amiloride.

    • Main Results:

    • A significant endogenous current was detected, exiting at the blastopore and entering elsewhere.
    • The inward current was abolished by Na+-free solutions, KCl, verapamil, ouabain, and amiloride, indicating Na+ dependence.
    • Amiloride also inhibited Li+-carried current, suggesting a shared transport mechanism.

    Conclusions:

    • Xenopus neurulae exhibit a Na+-dependent epithelial current during early development.
    • This current shares similarities with the amiloride-sensitive Na+ current found in adult frog skin.
    • The findings provide insights into early ion transport mechanisms during Xenopus embryogenesis.