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Increased biliary cholesterol secretion in alloxan diabetic mice
The Japanese Journal of Surgery
|March 1, 1984
Summary
Alloxan-induced diabetes in mice significantly elevates plasma and liver cholesterol, increasing bile cholesterol saturation and lithogenic index. Dietary cholesterol further impacts liver and bile lipid profiles differently than diabetes.
Area of Science:
- Biochemistry
- Metabolic Disorders
- Animal Models
Background:
- Diabetes mellitus is associated with altered lipid metabolism.
- Cholesterol homeostasis is complex and influenced by diet and disease states.
- Biliary lipid composition is crucial for preventing cholesterol gallstone formation.
Purpose of the Study:
- To investigate the effects of alloxan-induced diabetes and dietary cholesterol on plasma, liver, and biliary lipid profiles in mice.
- To determine how diabetes and high cholesterol diets influence the lithogenicity of bile.
- To elucidate potential differences in mechanisms regulating biliary cholesterol secretion between diabetic and cholesterol-fed states.
Main Methods:
- Mice were made diabetic using alloxan or fed a 0.5% cholesterol diet.
- Plasma, liver, and gallbladder bile were analyzed for cholesterol, phospholipid, and bile acid concentrations.
- Bile weight, lipid concentrations, cholesterol molar concentration ratio (mole percent), and lithogenic index were calculated.
Main Results:
- Alloxan-induced diabetes markedly increased plasma and liver cholesterol levels.
- Diabetic mice exhibited increased gallbladder bile weight and biliary lipid concentrations, with cholesterol and phospholipids rising more than bile acids.
- Dietary cholesterol increased liver cholesterol and biliary cholesterol/phospholipid concentrations, raising the lithogenic index, but affected bile acid composition differently than diabetes.
Conclusions:
- Alloxan-induced diabetes significantly increases bile cholesterol saturation and the lithogenic index in mice.
- The mechanisms driving increased biliary cholesterol secretion in diabetic mice appear distinct from those induced by a high-cholesterol diet.
- These findings highlight the complex interplay between diabetes, diet, and cholesterol metabolism in determining bile composition and gallstone risk.