A method for calibrating the gain of the electro-oculogram (EOG) using the optical properties of the eye

A method has been developed to measure the gain of the electro-oculogram (in volts per degree of eye rotation) which does not require the experimental subject to gaze at reference targets. The basis of the method is that the positional difference between the center of the entrance pupil and the corneal reflection of a light source is linearly related to the angle of eye rotation and is equal to zero when the pupillary axis of the eye is directed at the light source. The method has been validated in man and cat.