Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Lymphokine production in T-lymphocyte subsets defined by active E-rosette formation.

D S Robbins, E L Belden, P M Hoffman

    Scandinavian Journal of Immunology
    |June 1, 1982
    PubMed
    Summary

    Human T cells capable of active E rosette formation produce leucocyte migration inhibitory factor (LIF). T cells lacking this ability do not produce LIF, indicating a specific subset is responsible for LIF production.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Effect of protein supplementation on expression and distribution of urea transporter-B in lambs fed low-quality forage.

    Journal of animal science·2008
    Same author

    Immune response and serum immunoglobulin G concentrations in beef calves suckling cows of differing body condition score at parturition and supplemented with high-linoleate or high-oleate safflower seeds.

    Journal of animal science·2006
    Same author

    Expression of CA-125 by progestational bovine endometrium: prospective regulation and function.

    Reproduction (Cambridge, England)·2003
    Same author

    Evaluation of the BT-1 serum assay for breast cancer.

    Human antibodies·2000
    Same author

    Ingestion of Neospora caninum tissue cysts by Mustela species.

    International journal for parasitology·1999
    Same author

    Clinical analysis of the novel breast cancer serum assay BT1.

    Anticancer research·1999

    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • Human T-lymphocyte subsets play crucial roles in immune responses.
    • Leukocyte migration inhibitory factor (LIF) is a key mediator in cellular immunity.
    • Active E rosette formation is a method used to identify specific T-cell subsets.

    Purpose of the Study:

    • To investigate the capacity of human T-cell subsets, identified by active E rosette formation, to produce LIF.
    • To determine if LIF production is restricted to a particular T-cell subset.

    Main Methods:

    • Human T cells were separated into active rosette-forming (A+) and depleted (A-) subsets.
    • Subsets were stimulated with mitogen (concanavalin A) or specific antigen (tuberculin purified protein derivative).
    • LIF production was measured in response to stimulation.

    Related Experiment Videos

    Main Results:

    • A+ T cells produced LIF when stimulated with concanavalin A, while A- T cells did not.
    • A+ T cells from tuberculin-sensitive donors produced LIF in response to tuberculin purified protein derivative, whereas A- T cells did not.
    • These findings demonstrate a correlation between active E rosette formation and LIF production.

    Conclusions:

    • Human T-cell production of LIF is primarily associated with T cells exhibiting active E rosette formation.
    • This specific T-cell subset is responsible for mediating LIF production in response to immunological stimuli.