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[Antibiotic resistance and its transmissibility in strains of opportunistic enterobacteria]
Abstract:
Sensitivity of 39 opportunistic Enterobacteria strains was studied with respect to tetracycline, kanamycin, ampicillin, levomycetin, streptomycin and nevigramon. The strains were isolated from children with acute intestinal infections of obscure etiology. 38 isolates were resistant to one or more drugs. The highest number of the strains was resistant to kanamycin, ampicillin and levomycetin, i.e. 81.5, 84.2 and 84.2 per cent of the cultures respectively. Nevigramon proved to be the most effective in vitro (76.4 per cent of the sensitive strains). The ability of the strains to transfer the antibiotic resistance markers on conjugation was tested. Transmission of R plasmids was shown in 75 per cent of the isolates.
Insights
Most opportunistic Enterobacteria strains from children with intestinal infections showed resistance to common antibiotics like kanamycin and ampicillin. However, nevigramon demonstrated significant in vitro effectiveness, and 75% of strains could transfer antibiotic resistance markers.
Area of Science:
- Microbiology
- Infectious Diseases
- Antimicrobial Resistance
Background:
- Opportunistic Enterobacteria are a significant cause of nosocomial infections.
- Antibiotic resistance in Enterobacteria poses a growing global health threat.
- Understanding resistance patterns in pediatric intestinal infections is crucial.
Purpose of the Study:
- To determine the antibiotic sensitivity profiles of Enterobacteria strains isolated from children with acute intestinal infections.
- To investigate the prevalence of antibiotic resistance markers and their transferability via conjugation.
Main Methods:
- Antibiotic sensitivity testing was performed on 39 Enterobacteria isolates using tetracycline, kanamycin, ampicillin, levomycetin, streptomycin, and nevigramon.
- Conjugation experiments were conducted to assess the transfer of antibiotic resistance markers.
Main Results:
- 38 out of 39 isolates exhibited resistance to at least one antibiotic.
- High resistance rates were observed for kanamycin (81.5%), ampicillin (84.2%), and levomycetin (84.2%).
- Nevigramon showed the highest in vitro efficacy, with 76.4% of strains being sensitive.
- Antibiotic resistance markers were transferable via conjugation in 75% of the tested isolates.
Conclusions:
- Enterobacteria strains from pediatric intestinal infections display widespread multidrug resistance.
- Nevigramon represents a potentially effective therapeutic option against these resistant strains.
- The high rate of R plasmid transfer highlights the significant risk of further dissemination of antibiotic resistance.