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Gene ompR and regulation of microcin 17 and colicin e2 syntheses
Journal of Bacteriology
|November 1, 1982
Abstract:
The production of microcin 17 is controlled by plasmid pRYC17. Chromosomal mutants unable to produce a normal amount of microcin were isolated in Escherichia coli. One of the mutations maps in the ompR locus, indicating that an active OmpR product is required for the synthesis of microcin 17. The same conclusion was obtained for the synthesis of colicin E2. Therefore, two new functions of the regulatory gene ompR have been revealed.
Insights
The OmpR regulatory gene is essential for producing microcin 17 in Escherichia coli. This study reveals new functions for OmpR in microcin and colicin synthesis.
Area of Science:
- Microbiology
- Molecular Biology
- Genetics
Background:
- Microcin 17 is a bacteriocin produced by Escherichia coli, controlled by plasmid pRYC17.
- The regulatory mechanisms governing microcin production are not fully understood.
Purpose of the Study:
- To investigate the genetic control of microcin 17 production.
- To identify host factors involved in microcin 17 synthesis.
Main Methods:
- Isolation and characterization of chromosomal mutants of Escherichia coli unable to produce normal amounts of microcin 17.
- Genetic mapping of mutations affecting microcin production.
Main Results:
- A mutation in the ompR locus was identified in mutants deficient in microcin 17 production.
- The OmpR protein was found to be required for microcin 17 synthesis.
- Evidence suggests OmpR also regulates colicin E2 synthesis.
Conclusions:
- The OmpR regulatory gene plays a crucial role in the synthesis of microcin 17.
- This study uncovers two novel functions for the OmpR gene in bacteriocin production.