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[Studies on bacteriologic contamination of potassium chloride solution 25% in multidose containers regularly used on
Abstract:
The use of multidose containers poses the problem of in-use microbial contamination. In a clinical investigation sterility tests of 25% potassium chloride solution (w/v) in multidose containers have been performed in-use on two surgical wards. The contents of 80 vials used for 1, 3, 7 or 10 days (with registered withdrawals) were sterility tested with the aid of blood agar plates. None of the 80 bottles were contaminated. It has been shown that lege artis withdrawals of 25% potassium chloride solution (w/v) from multidose containers involves a very low risk of contamination. However, the theory that contaminants have been killed must not be excluded. In final laboratory tests, multidose containers of 25% sterile potassium chloride solution (w/v) were artificially contaminated. Test microorganisms were E. coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 and Staph. aureus ATCC 25923. Microbial control after 1 or 2 days storage showed absence or significant germ reduction with the experimental technique described.
Insights
Sterile potassium chloride solution in multidose containers showed no contamination in clinical use. Proper withdrawal techniques minimize microbial risk, though the solution may possess inherent antimicrobial properties.
Area of Science:
- Clinical Pharmacy
- Microbiology
- Sterilization and Disinfection
Background:
- Multidose containers present a risk of microbial contamination during use.
- Maintaining sterility of pharmaceutical solutions is critical in healthcare settings.
Purpose of the Study:
- To assess the in-use microbial contamination risk of 25% potassium chloride solution in multidose containers.
- To evaluate the effectiveness of proper withdrawal techniques in preventing contamination.
Main Methods:
- Clinical investigation involving sterility testing of 80 vials of 25% potassium chloride solution used in surgical wards.
- Blood agar plate method used for sterility testing over 1, 3, 7, and 10 days with recorded withdrawals.
- Laboratory tests involving artificial contamination of sterile solutions with E. coli, P. aeruginosa, and S. aureus.
Main Results:
- No microbial contamination was detected in any of the 80 clinically used vials.
- Laboratory tests showed absence or significant reduction of microbial growth after 1-2 days with the described technique.
- Withdrawals performed using lege artis (according to the art) principles demonstrated a very low contamination risk.
Conclusions:
- Leg artis withdrawals from multidose containers of 25% potassium chloride solution carry a minimal risk of microbial contamination.
- The solution itself may possess properties that inhibit or kill contaminating microorganisms.
- The findings support the safe in-use of multidose potassium chloride containers when proper aseptic techniques are followed.