[Redox enzymes in the corneal cells after low-temperature preservation (a cytochemical study)]

Activity of LDG, MDG, alpha-GFDG, NAD X H2DG and NADP X H2DG was studied in different type cells of rabbit cornea subjected to cryopreservation (-196 degrees C) in the presence of PEO-400. Fresh cornea was used for comparison. It is revealed that the enzyme activity in the represerved tissue changes: the level of cytochemical reaction increases with MDG and alpha-GFDG detection and decreases with NADP X H2DG detection. The difference in response to cooling may be attributed to unequal sensitivity of structural cell elements to cooling.