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Multiple mechanisms for expression of incompatibility by broad-host-range plasmid RK2
Journal of Bacteriology
|December 1, 1982
Summary
RK2 plasmid DNA exhibits incompatibility through multiple mechanisms. One mechanism involves the replication origin, while a second, IncP-1(II), is located in a specific DNA region and affects plasmid maintenance.
Area of Science:
- Molecular Biology
- Genetics
- Microbiology
Background:
- Plasmid incompatibility is a key factor in maintaining plasmid populations.
- The RK2 plasmid is a well-studied conjugative plasmid with complex regulatory mechanisms.
Purpose of the Study:
- To elucidate the molecular mechanisms underlying RK2 plasmid incompatibility.
- To identify and characterize novel determinants of incompatibility in RK2.
Main Methods:
- Cloning of RK2 DNA fragments into plasmid vectors.
- Analysis of plasmid inheritance and stability in the presence of different RK2 derivatives.
- Genetic mapping of incompatibility determinants.
Main Results:
- Two distinct mechanisms of RK2 incompatibility were identified.
- One determinant is linked to the origin of replication and requires an active RK2 replicon.
- A second determinant, IncP-1(II), located in the 54.1- to 56.4-kilobase region, functions independently of the RK2 replication system.
Conclusions:
- RK2 incompatibility is mediated by at least two distinct genetic elements.
- The IncP-1(II) determinant likely involves genes essential for plasmid maintenance and regulation of killing functions.