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Cloning and characterization of Escherichia coli K-12 regulator gene tyrR
Journal of Bacteriology
|December 1, 1982
Abstract:
The Escherichia coli K-12 regulator gene tyrR was cloned into the multicopy plasmid pBR322 from a lambda(Tn10)tyrR+ specialized transducing phage. Further subcloning localized the gene within a 2.1-kilobase region. Analysis of plasmid-coded proteins showed that the tyrR gene codes for a 63,000-dalton polypeptide.