A specific enzyme-linked immunosorbent assay for determination of bovine IgM: use in screening hybridoma cell lines

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H Ungar-Waron, I Davidson, Z Trainin

    Journal of Immunological Methods
    |September 17, 1982
    PubMed

    A highly specific enzyme-linked immunosorbent assay (ELISA) is described for determination of bovine IgM and use in screening IgM-secreting hybridoma cell lines. The advantages of this assay over the method of radioactive labelling of cells followed by immune precipitation, SDS-PAGE, fluorography and autofluorography are demonstrated.

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