Serologic grouping and sexual compatibility of airborne Cryptococcus neoformans

Through the use of Anderson air samples, 214 isolates of Cryptococcus neoformans were cultured from the air in a vacant tower in a large complex of buildings in Oklahoma City. The tower contained hundreds of pigeons, a massive amount of droppings, nests with eggs and young, dying and dead pigeons. All isolates were serotype A-D and self-sterile for the production of basidiospores. Among these 193 were of the "alpha" mating type, producing basidiospores when paired with "alpha" mating type. No isolates of "alpha" mating type were found. The findings imply that the infectious particles of C. neoformans in nature are relatively small, nonencapsulated yeast cells and not basidiospores.