Effect of phosphate omission on the glucose-induced insulin release in vitro in fed and fasted rats

Isolated perfused rat pancreases from fed and 20-h fasted rats were used to investigate the effect of phosphate omission on the insulin response to 16.7 mmol/l glucose. The insulin response to continuous glucose stimulation was biphasic. Both phases were significantly reduced when pancreases were perfused in the absence of extracellular phosphate in fasted, but not in fed rats.