A procedure for selecting specific areas of sections for comparative light and electron microscopic studies

Area of Science:

• Biotechnology
• Microscopy
• Cell Biology

Background:

• Correlative light and electron microscopy (CLEM) is crucial for high-resolution biological imaging.
• Existing CLEM techniques can be complex and time-consuming, requiring precise sample manipulation.

Purpose of the Study:

• To introduce a novel specimen marker for simplified and accurate CLEM.
• To enable precise targeting of specific cellular or tissue structures for correlative imaging.

Main Methods:

• Development of a new specimen marker attachable to a light microscope objective.
• Integration of the marker into the light microscopy workflow for area selection.
• Application of the technique in immunoelectronmicroscopy studies.

Main Results:

• The marker allows operators to examine and precisely encircle selected areas without objective changes.
• Demonstrated utility through two examples in immunoelectronmicroscopy.
• Facilitates seamless transition between light and electron microscopy for specific targets.

Conclusions:

• The new specimen marker significantly enhances the efficiency and precision of CLEM.
• This method provides a valuable tool for detailed ultrastructural analysis of biological samples.
• The technique is applicable to various research areas requiring high-resolution correlative imaging.