Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Membrane potential (delta psi) depolarizing agents inhibit maturation.

J M Pagès, C Lazdunski

    FEBS Letters
    |November 22, 1982
    PubMed
    Summary

    Phenethyl alcohol (PEA) treatment allowed precursor protein accumulation and processing. Colicins A and E1 inhibited this processing, indicating the electrical gradient is crucial for exported protein maturation in cells.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Cross-resistance between biocides and antimicrobials: an emerging question.

    Revue scientifique et technique (International Office of Epizootics)·2012
    Same author

    Physiological characterisation of the efflux pump system of antibiotic-susceptible and multidrug-resistant Enterobacter aerogenes.

    International journal of antimicrobial agents·2010
    Same author

    An AcrAB-mediated multidrug-resistant phenotype is maintained following restoration of wild-type activities by efflux pump genes and their regulators.

    International journal of antimicrobial agents·2009
    Same author

    Enterobacter gergoviae and the prevalence of efflux in parabens resistance.

    The Journal of antimicrobial chemotherapy·2006
    Same author

    Imipenem resistance in Salmonella enterica serovar Wien related to porin loss and CMY-4 beta-lactamase production.

    Antimicrobial agents and chemotherapy·2003
    Same author

    Import of colicins across the outer membrane of Escherichia coli involves multiple protein interactions in the periplasm.

    Molecular microbiology·2001

    Area of Science:

    • Cellular biology
    • Protein processing
    • Membrane biophysics

    Background:

    • Exported proteins require specific processing for maturation.
    • Phenethyl alcohol (PEA) can induce accumulation of precursor proteins.
    • Colicins are known to interact with the bacterial cytoplasmic membrane.

    Purpose of the Study:

    • To investigate the role of the electrical gradient in the cytoplasmic membrane on the maturation of exported proteins.
    • To determine if colicins affect the processing of accumulated precursor proteins.

    Main Methods:

    • Treatment of bacterial cells with phenethyl alcohol (PEA) to accumulate precursor proteins.
    • Exposure of PEA-treated cells to colicins A and E1.
    • Monitoring the processing of precursor proteins in the presence and absence of colicins.

    Main Results:

    • Precursor proteins accumulated in the envelope of PEA-treated cells.
    • Colicins A and E1 inhibited the processing of these accumulated precursor proteins.
    • Low concentrations of colicins (1 killing unit) were sufficient to inhibit processing.

    Conclusions:

    • The electrical gradient across the cytoplasmic membrane is essential for the maturation of exported proteins.
    • Colicin activity, which targets the electrical gradient, directly impacts protein processing.
    • This suggests a direct link between membrane potential and the post-translational modification of exported proteins.

    Related Experiment Videos