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A simple bile-disk method for the identification of Bacteroides fragilis
Microbiology and Immunology
|January 1, 1982
Summary
A new method uses oxgall-impregnated filter paper disks to easily identify Bacteroides fragilis. This bile resistance test offers a quick, economical, and reliable alternative for clinical microbiology labs.
Area of Science:
- Microbiology
- Clinical Diagnostics
- Bacteriology
Background:
- Accurate identification of Bacteroides fragilis is crucial in clinical microbiology.
- Existing methods for differentiating Bacteroides species can be time-consuming or complex.
Purpose of the Study:
- To develop a simple, rapid, and economical method for identifying Bacteroides fragilis.
- To evaluate the efficacy of oxgall-impregnated filter paper disks for this purpose.
Main Methods:
- Utilized oxgall-impregnated filter paper disks placed on Gifu anaerobic medium agar plates inoculated with bacterial lawns.
- Incubated plates under anaerobic conditions (hydrogen and carbon dioxide) at 35°C for 24 hours.
- Identified bile-resistant bacteria as Bacteroides fragilis.
Main Results:
- The oxgall disk method effectively separated Bacteroides fragilis from other Bacteroides species.
- An optimal bile salt concentration of 25 mg per disk was determined.
- Results closely mirrored those of the conventional tube method.
Conclusions:
- The oxgall-impregnated filter paper disk method is a simple, time-saving, economical, and clear technique.
- This method is suitable for routine daily use in clinical microbiology laboratories for Bacteroides fragilis identification.