Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cell separation using fluorescence emission anisotropy.

D J Arndt-Jovin, T M Jovin

    Progress in Clinical and Biological Research
    |January 1, 1976
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Elevated α-synuclein caused by SNCA gene triplication impairs neuronal differentiation and maturation in Parkinson's patient-derived induced pluripotent stem cells.

    Cell death & disease·2015
    Same author

    Alpha-Synuclein affects neurite morphology, autophagy, vesicle transport and axonal degeneration in CNS neurons.

    Cell death & disease·2015
    Same author

    Visualization of lipid-receptor interactions on single cells by time-resolved imaging fluorescence microscopy.

    Journal of fluorescence·2013
    Same author

    Effects of Oxidation Agents and Metal Ions on Binding of p53 to Supercoiled DNA.

    Journal of biomolecular structure & dynamics·2012
    Same author

    Minimizing light exposure with the programmable array microscope.

    Journal of microscopy·2010
    Same author

    Sensitivity analysis predicts that the ERK-pMEK interaction regulates ERK nuclear translocation.

    IET systems biology·2010

    Emission anisotropy measurements of fluorescent probes reveal cellular changes in living cells. This technique allows for high-speed cell separation based on structural and functional states for further analysis.

    Area of Science:

    • Biophysics
    • Cell Biology
    • Biochemistry

    Background:

    • Emission anisotropy of fluorescent probes is a sensitive indicator of cellular structural changes.
    • Previous limitations in measurement speed and cell separation hindered dynamic studies.
    • Understanding cellular functional states requires advanced analytical techniques.

    Purpose of the Study:

    • To develop and validate a method for measuring emission anisotropy in individual living cells at high throughput.
    • To utilize a multiparameter automated computer-controlled cell separator (MACCS) for cell isolation based on anisotropy.
    • To enable the study of dynamic cellular changes during differentiation and malignant transformation.

    Main Methods:

    • Utilizing fluorescent probes that interact with cell membranes.

    Related Experiment Videos

  • Performing single-cell emission anisotropy measurements at rates up to 10^3 per second.
  • Employing a multiparameter automated computer-controlled cell separator (MACCS) for cell sorting based on anisotropy and other physical signals.

    • Main Results:

    • Successful high-speed measurement of emission anisotropy in individual living cells.
    • Demonstrated cell separation based on anisotropy, reflecting cellular size, macromolecular content, and rotational mobility.
    • Isolated cells were viable, sterile, and suitable for subsequent outgrowth and biochemical analyses.

    Conclusions:

    • High-throughput emission anisotropy measurements coupled with MACCS provide a powerful tool for analyzing cellular states.
    • This method facilitates the study of dynamic cellular processes, including differentiation and malignant transformation.
    • The ability to isolate specific cell populations based on anisotropy opens new avenues for biochemical and functional studies.