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[S. typhimurium strains which are H2S negative in TSI medium]
Mikrobiyoloji Bulteni
|January 1, 1981
Summary
During an epidemic, Salmonella typhimurium strains initially negative for hydrogen sulfide (H2S) production were identified. Supplementing a specific medium with Pepton witte, meat extract broth, and sodium thiosulfate restored H2S production.
Area of Science:
- Microbiology
- Bacteriology
- Clinical Diagnostics
Context:
- An epidemic of Salmonella typhimurium occurred in a premature ward.
- 15 cases were diagnosed, with strains isolated from various clinical specimens including stool, blood, and joint material.
- The isolated S. typhimurium strains were initially H2S (TSI medium) negative.
Purpose:
- To investigate the H2S production capabilities of S. typhimurium strains isolated during an epidemic.
- To identify the essential components for H2S production in a modified medium (Braun-Ozek B Medium).
- To compare the efficacy of different sulfur sources for H2S production.
Summary:
- Salmonella typhimurium strains isolated from an epidemic were found to be H2S negative in standard TSI medium.
- When transferred to Braun-Ozek B Medium, these strains produced H2S, indicated by blackening on lead acetate paper.
- Component replacement studies revealed that Pepton witte + meat extract broth + Na2S2O3 . 5H2O were essential for H2S production in Braun-Ozek B Medium.
- Replacing Na2S2O3 . 5H2O with ferrous ammonium sulfate reduced and delayed H2S production.
Impact:
- This research identifies key nutritional and chemical factors required for H2S production by S. typhimurium.
- The findings contribute to understanding bacterial metabolism and optimizing diagnostic media for accurate Salmonella identification.
- This knowledge can aid in the development of more sensitive and specific laboratory tests for detecting S. typhimurium during outbreaks.