Related Experiment Video

Updated: Sep 20, 2026

Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry
11:08

Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry

Published on: May 5, 2017

Abnormalities of T cells in patients with ulcerative colitis. FACS analysis of peripheral lymphocytes

Y Shiratori, T Hirano, K Okumura

    Scandinavian Journal of Gastroenterology
    |November 1, 1982
    PubMed

    Heterologous antisera against human brain tissue and human embryonal thymocytes were used to distinguish the changes in lymphocyte subpopulations accompanying ulcerative colitis. Peripheral lymphocytes from normal individuals and from patients with ulcerative colitis were reacted with these heterologous antibodies, stained with fluoresceinated rabbit anti-human immunoglobulins, and analyzed by fluorescence-activated cell sorter. All of the fluorescence profiles of T cells in ulcerative colitis showed shifts with a marked increase in the population that had high fluorescence intensity. Time-course analysis by fluorescence-activated cell sorter revealed that the grade of fluorescence profile shift varied in accordance with the activity of the disease. Natural T-cell toxic autoantibody was found in 27 of the 35 patients with ulcerative colitis. Natural T-cell toxic autoantibodies from these patients reacted with 20% of peripheral T cells without Fc receptors. The abnormality of peripheral blood lymphocytes in ulcerative colitis may be caused by the production of autoantibodies.

    More Related Videos

    Isolation of CD4+ T-cells and Analysis of Circulating T-follicular Helper (cTfh) Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry07:39

    Isolation of CD4+ T-cells and Analysis of Circulating T-follicular Helper (cTfh) Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry

    Published on: January 7, 2019

    Analysis of Human T Cell Activity in an Allogeneic Co-Culture Setting of Pre-Treated Tumor Cells09:04

    Analysis of Human T Cell Activity in an Allogeneic Co-Culture Setting of Pre-Treated Tumor Cells

    Published on: March 7, 2025

    Related Concept Videos

    T Cell Types and Functions01:24

    T Cell Types and Functions

    When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
    Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
    Inflammatory Bowel Disease I: Ulcerative Colitis01:27

    Inflammatory Bowel Disease I: Ulcerative Colitis

    Introduction
    Inflammatory bowel disease, or IBD, encompasses a group of disorders characterized by chronic inflammation or ulceration of the gastrointestinal tract.
    Risk Factors
    The exact cause of IBD remains unclear, although it is believed to be due to a mix of genetic, environmental, microbial, and immune factors. Genetic factors are significant in determining susceptibility to IBD, with family history being a critical risk factor. Individuals with a first-degree relative who has IBD are at...
    Inflammatory Bowel Disease II: Ulcerative Colitis01:20

    Inflammatory Bowel Disease II: Ulcerative Colitis

    Ulcerative colitis is a chronic inflammatory disorder of the colon characterized by continuous mucosal inflammation that typically begins in the rectum and extends proximally in a uniform pattern. Its pathogenesis involves a complex interplay of genetic predisposition, immune dysregulation, and environmental influences. These factors converge to impair the colon’s epithelial defenses and promote an exaggerated inflammatory response against luminal contents.Breakdown of the Mucosal BarrierA...