Radiodetoxified endotoxin induced lysosomal enzyme liberation and tolerance

Beta-glucuronidase and cathepsin D activity was measured in rabbit sera. A significant increase in beta-glucuronidase activity was found following the intravenous application of a high dose of the parent endotoxin. The enzyme liberating effect of endotoxins irradiated by 50, 100, and 150 kGy was much weaker. Enzyme assays also confirmed that, independent of the irradiation, radiodetoxified endotoxins retain their endotoxin tolerance inducing effect.