Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Localisation of mini-Mu in its replication intermediates.

A Résibois, M Colet, A Toussaint

    The EMBO Journal
    |January 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Radiation dose comparison of conventional radiography and photon-counting CT for ventriculoperitoneal shunt imaging: A phantom study.

    Radiography (London, England : 1995)·2026
    Same author

    Combining NSAM and CPC concentrations to determine airborne nanoparticle count median diameter: Application to various laboratory and workplace aerosols.

    Journal of occupational and environmental hygiene·2018
    Same author

    Global functional diversity of freshwater fish is concentrated in the Neotropics while functional vulnerability is widespread.

    Scientific reports·2016
    Same author

    X-chromosome inactivation in female patients with Fabry disease.

    Clinical genetics·2015
    Same author

    [Management of acute iliac thrombosis in a pregnancy at term: case discussion and review of literature].

    Revue medicale de Bruxelles·2013
    Same author

    Ultrasound-guided perineural circumferential median nerve block in carpal tunnel syndrome.

    Anaesthesia·2013

    Mu delta 26 sequences were found within DNA structures during replication. These findings provide evidence that these structures are key intermediates in mini-Mu replication processes.

    Area of Science:

    • Molecular Biology
    • Genetics
    • Microbiology

    Background:

    • Transposable elements, such as bacteriophage Mu, are mobile DNA sequences that can change their position within a genome.
    • Understanding the mechanisms of transposition is crucial for comprehending genome dynamics and evolution.
    • Previous models have proposed various intermediate structures during transposition, but direct evidence has been limited.

    Purpose of the Study:

    • To investigate the structural intermediates involved in Mu delta 26 replication.
    • To provide experimental evidence supporting the role of specific DNA structures in mini-Mu transposition.
    • To correlate observed DNA structures with theoretical models of transposition.

    Main Methods:

    • Localization of Mu delta 26 sequences within specific DNA structures using molecular techniques.

    Related Experiment Videos

  • Analysis of DNA structures formed during Mu delta 26 replication, including keys, pending keys, dumb-bells, partially fused circles, and asymmetrical forks.
  • Comparison of observed structures with predictions from existing transposition models.

    • Main Results:

    • Mu delta 26 sequences were successfully located within various DNA structures observed during Mu delta 26 replication.
    • These structures, including keys and partially fused circles, are implicated as mini-Mu replication intermediates.
    • The findings offer direct evidence supporting the existence of these intermediates.

    Conclusions:

    • The identified DNA structures are confirmed as critical intermediates in the Mu delta 26 replication process.
    • This research strengthens the understanding of transposition mechanisms in prokaryotes.
    • The study provides a foundation for further exploration of transposition models and their validation.