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[Optimizing the fibrinogen-thrombin adhesive system]
A Lindner1, M Elliott, F Holzer
1Institut für allgemeine und experimentelle Pathologie, Universität Wien.
Wiener Klinische Wochenschrift. Supplementum
|January 1, 1980
Summary
Optimizing the fibrinogen-thrombin adhesive system involves adjusting fibrinogen and thrombin concentrations, component ratios, and adding Factor XIII and calcium ions. These factors significantly enhance clot firmness for improved adhesive performance.
Area of Science:
- Biochemistry
- Biomaterials Science
Context:
- Fibrinogen-thrombin systems are crucial biomaterials for tissue adhesion.
- Optimizing clot firmness is essential for effective application in surgical settings.
Purpose:
- To determine optimal conditions for enhancing the firmness of fibrinogen-thrombin clots.
- To investigate the impact of fibrinogen and thrombin concentrations, component ratios, and additives on clot properties.
Summary:
- Investigated factors influencing fibrinogen-thrombin clot firmness, including fibrinogen/thrombin concentrations, volume ratios, diffusion time, Factor XIII, and calcium ions.
- Established optimal concentrations: 0.86 ml fibrinogen solution (10% clottable), 100 E thrombin, 90 E Factor XIII (30 mg), and 250 mMol Ca++ per ml.
- A 6:1 volume ratio of fibrinogen cryoprecipitate to a solution of thrombin, Factor XIII, and calcium ions yielded the best results.
Impact:
- Provides specific parameters for preparing a more robust fibrinogen-thrombin adhesive.
- Enhances understanding of biomaterial formulation for improved hemostatic and sealing applications.
- Findings can guide the development of more effective surgical glues and sealants.