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Abstract:
Incubation of linoleic acid with partially purified lipoxygenase from rice germ yielded a ratio of 9- to 13-hydroperoxides of linoleic acid of 97:3 as measured by high performance liquid chromatography. Under similar conditions, hematin gave the 9- to 13-hydroperoxides at a ratio of 51:49, and soybean lipoxygenase-a at 9:91. Infrared spectral analysis revealed cis-trans configuration to predominate in the reaction products with the rice germ enzyme as was with the soybean enzyme.