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Human renal renin. Complete purification and characterization.

H Yokosawa, L A Holladay, T Inagami

    The Journal of Biological Chemistry
    |April 25, 1980
    PubMed
    Summary
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    Researchers purified human renin from kidneys, achieving high purity and specific activity. This pure human renin exhibits unique properties distinct from other mammals, with specific antibodies showing minimal cross-reactivity.

    Area of Science:

    • Biochemistry
    • Enzymology
    • Physiology

    Background:

    • Human renin is a critical enzyme in the renin-angiotensin-aldosterone system, regulating blood pressure.
    • Previous studies lacked highly purified human renin for detailed characterization.
    • Understanding human renin's properties is vital for hypertension research.

    Purpose of the Study:

    • To achieve complete purification of human renin.
    • To characterize the biochemical and physical properties of pure human renin.
    • To compare human renin with renin from other mammalian species.

    Main Methods:

    • Purification of human renin from noncancerous, autopsied kidneys using multiple chromatography steps.
    • Purity assessment via polyacrylamide gel electrophoresis, isoelectric focusing, and analytical ultracentrifugation.

    Related Experiment Videos

  • Characterization of molecular weight, isoelectric point, and substrate specificity.

    • Main Results:

    • Achieved a 480,000-fold purification of human renin with a specific activity of 950 Goldblatt units/mg.
    • Determined molecular weight (40,000 by sedimentation equilibrium) and isoelectric point (pH 5.7).
    • Human renin demonstrated unique properties, including carbohydrate presence and distinct substrate pH optima compared to other species, with specific antibodies showing low cross-reactivity.

    Conclusions:

    • Successfully purified and characterized human renin, establishing it as a distinct enzyme.
    • The unique properties of human renin suggest species-specific roles in physiological regulation.
    • This purified enzyme and its specific antibodies are valuable tools for further research into the renin-angiotensin system.