Autoradiographic localization of carbonic anhydrase in the developing chorioallantoic membrane

Carbonic anhydrase was localized in the chorioallantoic membrane with labeled inhibitor autoradiography of 3H-acetazolamide at 11, 14 and 19 days of incubation. At 11 days carbonic anhydrase was present in low amounts only in the undifferentiated ectoderm cells. At 14 and 19 days, the enzyme was found in increased amounts in all three germ layers of the chorioallantois. In the chorionic ectoderm the villous cavity cells contained the highest level of carbonic anhydrase. This finding lends support to the theory of H+ production to solubilize the CaCO3 of the egg shell. Sinus covering cells showed a considerable lower concentration of the enzyme than did villous cavity cells. Carbonic anhydrase in these cells may be multifunctional, assisting in calcium transport, subserving HCO3- transport from egg shell to blood, and supporting gaseous exchange. In the allantoic endoderm carbonic anhydrase was found in granule-rich cells and might be involved in the transport of Na+ and Cl- ions from allantoic fluid into the blood. The enzyme in the undifferentiated endoderm cells may have a respiratory function. In the mesoderm carbonic anhydrase was detected in the endothelium and pericytes of blood vessels where it is interpreted to support respiratory function.