Complete HLA and Bf typing in families. I. Analysis of MLC responses and LD typing

A triple normalization protocol for the analysis of MLC responses was evaluated using data obtained in more than 1700 triplicate reactions between the members of 17 families. The results show the method to be consistent with immunogenetic expectations. Typing responses to HTC, however, may have values identical with those of some weak one-haplotype responses and often can only be resolved by segregationanalysis.