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Dose response with sensitizing antibody in EA rosette formation
With the exception of the EA rosette test using SRBC, all other assay systems using BRBC and HRBC showed a significant dose response in rosette-forming cell count when the sensitizing antibody was further diluted. The percent of rosette-forming cell counts of the EA assay with SRBC, however, was unaffected by dilutions of antiserum even at exceedingly high levels. The present results suggest using red cells, which are nonreactive with T lumphocytes for the assay of B lymphocytes, and the use of antibody for sensitization of red blood cells must be adjusted for the optimal dilution necessary for proper EA rosette formation.
With the exception of the EA rosette test using SRBC, all other assay systems using BRBC and HRBC showed a significant dose response in rosette-forming cell count when the sensitizing antibody was further diluted. The percent of rosette-forming cell counts of the EA assay with SRBC, however, was unaffected by dilutions of antiserum even at exceedingly high levels. The present results suggest using red cells, which are nonreactive with T lumphocytes for the assay of B lymphocytes, and the use of antibody for sensitization of red blood cells must be adjusted for the optimal dilution necessary for proper EA rosette formation.