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Calcium in epithelial cell contraction.

H C Lee, N Auersperg

    The Journal of Cell Biology
    |May 1, 1980
    PubMed
    Summary
    This summary is machine-generated.

    Epithelial cell contraction involves increased intracellular calcium ([Ca]i). This study shows that extracellular calcium influx is essential for these contractions, as demonstrated in cultured carcinoma cells.

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    Area of Science:

    • Cell Biology
    • Biochemistry
    • Physiology

    Background:

    • Epithelial morphogenesis relies on microfilament-mediated cellular contractions.
    • Similar contractions are observed in cultured C-4II carcinoma cells, sensitive to cytochalasin B.

    Purpose of the Study:

    • To compare intracellular calcium levels ([Ca]i) in contracted versus control epithelial cell cultures.
    • To investigate the dependence of epithelial cell contraction on extracellular calcium influx.

    Main Methods:

    • Atomic absorption spectrophotometry was used to measure total intracellular calcium ([Ca]i).
    • Monolayer fragments of C-4II carcinoma cells were used to assess contraction.
    • Lanthanum was used to define [Ca]i as non-displaceable calcium.

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    Main Results:

    • Cellular contractions, initiated by detachment, caused an immediate increase in [Ca]i.
    • Calcium influx was required for contraction, as shown by inhibition in Ca-free medium and by papaverine, lanthanum, and D-600.
    • The Ca ionophore A23187 showed inhibitory effects at higher concentrations.

    Conclusions:

    • Direct evidence supports elevated intracellular calcium ([Ca]i) during epithelial cell contraction.
    • Extracellular calcium influx is a critical requirement for epithelial cell contraction.