[Localization of polyphosphates in cells of microorganisms using 31-P-NMR-145, 75 MHz of high resolution]

Using the method of 31P-NMR of high resolution at 145,78 MHz the presence of mobile inorganic polyphosphates in the cells of actinomycetes (Mycobacterium smegmatis), yeasts (Candida albicans and Endomyces magnusii) and bacteria (E. coli) was established. A considerable increase in the intensity and a low field shift of the polyphosphate signal after addition of EDTA to the M. Smegmatis cells can be indicative of possible localization of inorganic polyphosphates in the periplasmic space. The lack of effect of exogenous EDTA and Mn2+ on the polyphosphate signal in the spectrum of E. magnusii cells is probably due to localization of polyphosphates inside the cells. A comparison of 31P-NMR spectra of living cells and bacterial extracts of Staphylococcus aureus, Micrococcus lysodeikticus, Bacillus antracoides, B. megaterium and Salmonella typhimurium is given.