Abnormal lysozyme production of peritoneal macrophages from mastocytoma P-815 bearing C3D2F1-mice
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Medical Microbiology and Immunology
|February 1, 1980
Abstract:
Sodium thioglycollate-induced peritoneal macrophages from C3D2F1 mice were examined for their ability to produce and secrete lysozyme in vitro. Lysozyme was quantitated by use of a "lysoplate assay", in which lyophilized Micrococcus lysodeicticus suspended in agar were lysed by the enzyme. The ability to secrete lysozyme decreased in mice bearing a subcutaneous or intraperitoneal mastocytoma P-815 with progressive tumour growth.
Insights
Sodium thioglycollate-induced macrophages in mice showed reduced lysozyme secretion with tumor growth. This study investigated the impact of mastocytoma P-815 on macrophage function and enzyme production.
Area of Science:
- Immunology
- Biochemistry
Background:
- Macrophages play a crucial role in innate immunity.
- Lysozyme is a key enzyme involved in bacterial lysis and immune defense.
Purpose of the Study:
- To investigate the effect of mastocytoma P-815 tumor growth on the production and secretion of lysozyme by peritoneal macrophages.
- To quantify lysozyme levels in macrophages from tumor-bearing mice.
Main Methods:
- Peritoneal macrophages were induced using sodium thioglycollate in C3D2F1 mice.
- Lysozyme production and secretion were assessed in vitro using a "lysoplate assay" with Micrococcus lysodeicticus.
- Mice were inoculated with subcutaneous or intraperitoneal mastocytoma P-815.
Main Results:
- A progressive decrease in the ability of macrophages to secrete lysozyme was observed in mice with growing mastocytoma P-815.
- Tumor burden correlated with reduced lysozyme secretion capacity.
Conclusions:
- Mastocytoma P-815 tumor growth impairs the functional capacity of peritoneal macrophages, specifically their lysozyme secretion.
- This impairment may have implications for the host's immune defense against bacterial infections during tumor progression.