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Fragments and subclasses of IgG in eluates of human malignant tissues
Eluates of 13 malignant tumours were prepared at 56 degrees C using the continuous flow technique. By using immunodiffusion techniques, 50--80 per cent of the IgG detected was found to be of the IgG1 subclass. The ratio of Ig/kappa to Ig/lambda was similar in eluates and in the corresponding extracts, and this ratio was similar to that obtained using pooled human serum. This indicates a normal distribution of IgG subclasses in the eluates. Besides whole IgG, the eluates and corresponding extracts contained fragments of IgG. This was revealed by using sodium dodecyl sulphate electrophoresis (SDS-PAGE). However, since parts of the IgG associated with human malignant tumours can be non-specifically bound, and since fragments of IgG was found in extracts and eluates of normal tissues, although to a lesser degree than in those of malignant tissue, no conclusive evidence was obtained that the malignant tissue could degrade Ig. SDS-PAGE of extracts and eluates of malignant tissues showed 2--3 constant bands not detected in isolated IgG or in extracts and eluates of most of the normal tissues. These bands were not identified.
Eluates of 13 malignant tumours were prepared at 56 degrees C using the continuous flow technique. By using immunodiffusion techniques, 50--80 per cent of the IgG detected was found to be of the IgG1 subclass. The ratio of Ig/kappa to Ig/lambda was similar in eluates and in the corresponding extracts, and this ratio was similar to that obtained using pooled human serum. This indicates a normal distribution of IgG subclasses in the eluates. Besides whole IgG, the eluates and corresponding extracts contained fragments of IgG. This was revealed by using sodium dodecyl sulphate electrophoresis (SDS-PAGE). However, since parts of the IgG associated with human malignant tumours can be non-specifically bound, and since fragments of IgG was found in extracts and eluates of normal tissues, although to a lesser degree than in those of malignant tissue, no conclusive evidence was obtained that the malignant tissue could degrade Ig. SDS-PAGE of extracts and eluates of malignant tissues showed 2--3 constant bands not detected in isolated IgG or in extracts and eluates of most of the normal tissues. These bands were not identified.