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A simple assay technique for pancreatic lipase.
The American Journal of Digestive Diseases
|May 1, 1978
Summary
A new, affordable method for measuring pancreatic lipase during duodenal aspiration is presented. This simple assay provides results comparable to existing methods, enhancing pancreatic function diagnostics.
Area of Science:
- Biochemistry
- Gastroenterology
- Clinical Diagnostics
Background:
- Pancreatic function tests are crucial for diagnosing exocrine pancreatic insufficiency.
- Duodenal aspiration is a common method for collecting pancreatic fluid.
- Accurate measurement of pancreatic enzymes, like lipase, is essential for reliable diagnosis.
Purpose of the Study:
- To describe a simple, inexpensive procedure for quantifying pancreatic lipase activity.
- To evaluate the efficacy of this new method compared to existing assays.
- To recommend the inclusion of pancreatic lipase assay in routine duodenal aspiration protocols.
Main Methods:
- The described method involves diluting samples with an albumin solution.
- Incubation with a commercial substrate mixture for 2 hours.
- Manual titration using a burette to determine lipase activity.
Main Results:
- The manual titration method yielded results comparable to the established pH-stat assay.
- The procedure is noted for its simplicity and low cost.
- Demonstrated reliability for clinical diagnostic use.
Conclusions:
- The developed procedure offers a viable and cost-effective way to measure pancreatic lipase.
- Incorporating this pancreatic lipase assay into duodenal aspiration protocols can improve diagnostic accuracy.
- This method provides a valuable tool for assessing pancreatic function.