Nucleotide sequence of initiator tRNA from Bacillus subtilis

Initiator methionine tRNA, tRNAfMet, was purified from Bacillus subtilis W168 by the use of two column chromatographies on DEAE-Sephadex A-50 and BD-cellulose. The nucleotide sequence was determined to be pC-G-C-G-G-G-G-U-G-G-A-G-C-A-G-U-U-C-G-G-D-A-G-C-U-C-G-U-C-G-G-G-C-U-C-A-U-A-A-C-C-C-G-A-A-G-G-U-C-G-C-A-G-G-T-psi-C-A-A-A-U-C-C-U-G-C-C-C-C-C-G-C-A-A-C-C-AOH. This TRNAfMet exhibited a melting temperature 8 degrees C lower than that of E. coli tRNAfMet in the presence of 0.01 M magnesium acetate.