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Postmortem biochemical changes in canine blood.

P Schoning, A C Strafuss

    Journal of Forensic Sciences
    |April 1, 1980
    PubMed
    Summary
    This summary is machine-generated.

    Postmortem blood analysis reveals stable urea nitrogen, calcium, and protein levels. Potassium, creatinine, and phosphorus increase over time, while sodium, chloride, and carbon dioxide decrease, aiding in estimating time of death in dogs.

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    Area of Science:

    • Veterinary Forensics
    • Clinical Pathology
    • Biochemistry

    Background:

    • Accurate postmortem interval (PMI) estimation is crucial in veterinary forensics.
    • Blood chemistry parameters can offer insights into the postmortem changes.
    • Understanding the stability and degradation patterns of various analytes is essential.

    Purpose of the Study:

    • To evaluate the stability of key blood chemical analytes at different postmortem temperatures and intervals.
    • To determine the diagnostic significance of these analytes for estimating the time of death in canine cases.
    • To identify reliable biomarkers for PMI determination in dogs.

    Main Methods:

    • Analysis of antemortem and postmortem canine blood samples (n=60).
    • Measurement of electrolytes (sodium, chloride, potassium), metabolites (urea nitrogen, glucose, creatinine), minerals (calcium, phosphorus), proteins (total protein, albumin), and carbon dioxide.

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  • Incubation of samples at controlled temperatures (4°C, 20°C, 37°C) and assessment at various postmortem intervals (3, 6, 12, 48 hours).

    • Main Results:

    • Blood urea nitrogen, calcium, and total protein levels demonstrated stability postmortem.
    • Potassium, creatinine, and phosphorus concentrations showed a significant increase with advancing postmortem intervals.
    • Sodium, chloride, and total carbon dioxide levels exhibited a notable decrease over time.

    Conclusions:

    • Blood urea nitrogen, calcium, and total protein are reliable indicators of diagnostic significance postmortem.
    • Changes in potassium, creatinine, phosphorus, sodium, chloride, and carbon dioxide levels can be utilized to estimate the postmortem interval in dogs.
    • Glucose levels provided limited value for PMI estimation.