Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A sensitive enzyme immunoassay for plasma cortisol.

G Kominami, I Fujisaka, A Yamauchi

    Clinica Chimica Acta; International Journal of Clinical Chemistry
    |May 9, 1980
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Development of a virtual reality simulator for training canine endotracheal intubation technique and evaluation of the educational impacts.

    Veterinary journal (London, England : 1997)·2024
    Same author

    The evolution of cooperation by negotiation in a noisy world.

    Journal of evolutionary biology·2016
    Same author

    Post-operative swallowing in multiple system atrophy.

    European journal of neurology·2015
    Same author

    Y chromosome haplogroup D2a1 is significantly associated with high levels of luteinizing hormone in Japanese men.

    Andrology·2015
    Same author

    Maternal IgG avidity, IgM and ultrasound abnormalities: combined method to detect congenital cytomegalovirus infection with sequelae.

    Journal of perinatology : official journal of the California Perinatal Association·2013
    Same author

    Involvement of autoimmunity to REG, a regeneration factor, in patients with primary Sjögren's syndrome.

    Clinical and experimental immunology·2013

    A new enzyme immunoassay for plasma cortisol offers enhanced sensitivity, detecting levels as low as 1 microgram/dl. While slightly more sensitive than radioimmunoassay, its precision is currently lower.

    Area of Science:

    • Biochemistry
    • Analytical Chemistry
    • Endocrinology

    Background:

    • Plasma cortisol measurement is crucial for diagnosing adrenal function disorders.
    • Enzyme immunoassays offer a non-radioactive alternative to traditional radioimmunoassays.
    • Previous enzyme immunoassays for cortisol lacked sufficient sensitivity for clinical applications.

    Purpose of the Study:

    • To develop and characterize a sensitive enzyme immunoassay for plasma cortisol.
    • To compare the sensitivity and precision of the developed enzyme immunoassay with radioimmunoassay.

    Main Methods:

    • Utilized a cortisol-beta-D-galactosidase conjugate as the labelled antigen.
    • Employed 4-methylumbelliferyl-beta-D-galactoside as the fluorescence substrate.
    • Compared performance metrics against a radioimmunoassay using 3H-labelled cortisol.

    Related Experiment Videos

    Main Results:

    • Achieved a minimal detectable level of 10 pg/tube (1 microgram/dl) for the enzyme immunoassay.
    • Demonstrated slightly higher sensitivity compared to radioimmunoassay.
    • Exhibited lower precision compared to radioimmunoassay.

    Conclusions:

    • The developed enzyme immunoassay provides a highly sensitive method for plasma cortisol quantification.
    • Further optimization may be needed to match the precision of radioimmunoassay.
    • This assay represents a significant advancement in non-radioactive cortisol detection.