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Related Experiment Videos

Stable acid phosphatase: I. Demonstration and distribution.

F M Eggert, J P Germain

    Histochemistry
    |January 1, 1980
    PubMed
    Summary

    A stable form of acid phosphatase (SAPhase) acts as a reliable cell marker for mesenchymal cells involved in digestion. Its presence indicates an association with lysosomes and digestive processes in various cell types.

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    Area of Science:

    • Histochemistry
    • Cell Biology
    • Biochemistry

    Background:

    • Acid phosphatases are enzymes involved in cellular digestion.
    • A specific stable form, SAPhase, was identified.
    • Its utility as a cell marker was previously limited.

    Purpose of the Study:

    • To characterize a highly stable form of acid phosphatase (SAPhase).
    • To evaluate SAPhase as a cell marker for specific mesenchymal cell types.
    • To investigate the cellular localization and properties of SAPhase.

    Main Methods:

    • pH-dependent reactivation of acid phosphatase.
    • Histochemical staining of tissue sections.
    • Use of formaldehyde fixation and non-aqueous fixatives.
    • Demineralization in acidic buffers.

    Main Results:

    • A highly stable acid phosphatase (SAPhase) was demonstrated in macrophages, giant cells, osteoclasts, chondrocytes, bone-lining cells, and eosinophils.
    • SAPhase exhibited stability after fixation and demineralization, unlike epithelial acid phosphatases.
    • Granular staining and non-aqueous fixative use suggest SAPhase is lysosome-associated.
    • High SAPhase levels were found in mesenchymal cells engaged in digestion.

    Conclusions:

    • SAPhase is a valuable and stable cell marker for mesenchymal cells involved in digestive processes.
    • The enzyme's properties suggest a lysosomal association and role in intra/extracellular digestion.
    • SAPhase highlights an interrelationship between mononuclear and multinuclear cells in digestive functions.

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