Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

pH equilibration in human erythrocyte suspensions.

A M Critz, E D Crandall

    The Journal of Membrane Biology
    |May 23, 1980
    PubMed
    Summary

    Human red blood cells rapidly equilibrate pH by transporting both hydrogen and hydroxyl ions across their membranes. Anion exchange inhibitors significantly reduce this ion flux, indicating a key role in pH regulation.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    High gene flow due to pelagic larval dispersal among South Pacific archipelagos in two amphidromous gastropods (Neritomorpha: Neritidae).

    Heredity·2009
    Same author

    Phenotype-dependent synthesis of transferrin receptor in rat alveolar epithelial cell monolayers.

    Cell and tissue research·2003
    Same author

    Re-evaluating the Na(+) conductance of adult rat alveolar type II pneumocytes: evidence for the involvement of cGMP-activated cation channels.

    The Journal of physiology·2001
    Same author

    Vesicular stomatitis virus G-pseudotyped lentivirus vectors mediate efficient apical transduction of polarized quiescent primary alveolar epithelial cells.

    Journal of virology·2001
    Same author

    A useful in vitro model for transport studies of alveolar epithelial barrier.

    Pharmaceutical research·2001
    Same author

    Alveolar epithelial transport. Basic science to clinical medicine.

    American journal of respiratory and critical care medicine·2001

    Area of Science:

    • Physiology
    • Biochemistry
    • Membrane Transport

    Background:

    • Human red blood cells play a crucial role in maintaining acid-base balance.
    • Understanding the mechanisms of pH equilibration in erythrocytes is vital for physiological studies.

    Purpose of the Study:

    • To investigate the rate and mechanisms of pH equilibration in human red blood cell suspensions.
    • To determine the flux of hydrogen (H+) and hydroxyl (OH-) ions across the red cell membrane under varying extracellular pH conditions.

    Main Methods:

    • Utilized a stopped-flow rapid reaction apparatus for precise kinetic measurements.
    • Measured extracellular pH changes in CO2-free erythrocyte suspensions after rapid mixing with solutions of extreme pH.
    • Calculated ion flux and velocity constants from the initial rate of pH change (dpH/dt).

    Main Results:

    • Increased deviation of extracellular pH from 7.3 resulted in greater absolute flux of H+ and/or OH- ions.
    • Pretreatment with SITS (4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonic acid), an anion exchange inhibitor, significantly reduced ion flux across the entire pH range.
    • Valinomycin, a potassium ionophore, had no measurable effect on pH equilibration.

    Conclusions:

    • Both H+ and OH- ions likely cross the red cell membrane during pH equilibration, with the dominant species depending on the extracellular pH gradient.
    • The erythrocyte membrane's rapid anion exchange pathway appears to facilitate the transport of both H+ and OH- ions.
    • Anion transport mechanisms are critical for CO2-free pH equilibration in human red blood cells.

    Related Experiment Videos