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High-frequency conjugation associated with Streptococcus lactis donor cell aggregation.

M J Gasson, F L Davies

    Journal of Bacteriology
    |September 1, 1980
    PubMed
    Summary
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    Researchers identified variants of the Streptococcus lactis 712 lactose plasmid with enhanced conjugal transfer capabilities. These variants also displayed an unusual cell aggregation phenotype, suggesting a link between plasmid transfer and bacterial behavior.

    Area of Science:

    • Microbiology
    • Genetics
    • Molecular Biology

    Background:

    • Conjugal transfer is a key mechanism for horizontal gene exchange in bacteria.
    • The Streptococcus lactis 712 lactose plasmid mediates lactose metabolism and can be transferred between bacterial cells.
    • The natural frequency of this plasmid's conjugal transfer is typically low.

    Purpose of the Study:

    • To investigate the genetic basis of conjugal transfer in Streptococcus lactis 712.
    • To identify and characterize variants of the lactose plasmid with improved transfer efficiency.
    • To explore the relationship between plasmid transfer ability and bacterial cell behavior.

    Main Methods:

    • Selection of Streptococcus lactis 712 lactose plasmid variants.
    • Assays to quantify conjugal transfer frequency.

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  • Phenotypic analysis of bacterial cell aggregation.

    • Main Results:

    • Identification of plasmid variants with significantly increased donor abilities.
    • Observation of an unusual cell aggregation phenotype associated with these high-transfer variants.
    • Demonstration that modifications to the lactose plasmid can enhance its conjugal transfer frequency.

    Conclusions:

    • The study successfully generated and characterized Streptococcus lactis 712 lactose plasmid variants with enhanced conjugal transfer.
    • The observed cell aggregation phenotype suggests a potential link between plasmid transfer mechanisms and bacterial interactions.
    • Further research into these variants could elucidate novel aspects of bacterial conjugation and plasmid evolution.