[ATP-dependent citrate lyase in the green phototrophic bacterium, Chlorobium limicola]

Cell-free extracts of the green sulfur bacterium Chlorobium limicola forma thiosulfatophilum, strains 1C and L, grown autotrophically and in media with organic substances were shown to cleave citrate with the formation of oxaloacetate and acetyl-CoA only when ATP, CoA and Mg2+ were added. These results suggest that C. limicola f. thiosulfatophilum produces ATP-linked citrate lyase (E. C. 4.1.3.8) which may play an important role in the carbon metabolism of this bacterium.