Genetic mapping of the G101 bacteriophage (Pseudomonas aeruginosa) by temperature-sensitive mutations

Temperature-sensitive (ts mutations of the G101 phage were isolated after mutagesis with hydroxylamine. A complementation analysis of 61 ts mutants showed that these mutants may be divided into a least 12 complementation groups. Two ts mutants probably originated in genes which control lytic functions of the G101 phage. It was shown by three factor crosses that all of the 12 ts mutations tested are localized on that side of the "c" region where the probable cI repressor gene is positioned. Seven ts mutations is closely linked to the cI26 clear marker, three exhibit a closer linkage and two do not exhibit any linkage with cI. All mutations isolated until now can be arrange linearly. According to the present knowledge the preliminary genetic map of the G101 phage is linear.