Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Gentamicin bioautography assay vs. the microbiological disk test.

A Rosner, H Aviv

    The Journal of Antibiotics
    |June 1, 1980
    PubMed
    Summary

    Bioautography offers a more sensitive method for measuring gentamicin concentrations compared to the traditional disk test. This technique produces significantly larger inhibition zones, enhancing quantitative analysis of antibiotic levels.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Blood group A epitopes do not facilitate entry of SARS-CoV-2.

    Journal of internal medicine·2021
    Same author

    Okra (Hibiscus esculentus)-A New Host of Turnip mosaic virus in Israel.

    Plant disease·2019
    Same author

    A New Pathotype of Pepper mild mottle virus (PMMoV) Overcomes the L<sup>4</sup> Resistance Genotype of Pepper Cultivars.

    Plant disease·2019
    Same author

    First Report of Recombinant Potato virus Y Strains Infecting Potato in Jordan.

    Plant disease·2019
    Same author

    Comparing the efficacy of three techniques to reduce isoagglutinin titers in AB0 incompatible kidney transplant recipients.

    Atherosclerosis. Supplements·2017
    Same author

    Methodological and clinical aspects of alloimmunization after granulocyte transfusion in patients undergoing allogeneic stem cell transplantation.

    Tissue antigens·2014

    Area of Science:

    • Pharmacology
    • Analytical Chemistry
    • Microbiology

    Background:

    • Accurate quantification of antibiotics like gentamicin is crucial for therapeutic efficacy and safety.
    • Traditional methods such as the disk diffusion assay have limitations in sensitivity.

    Purpose of the Study:

    • To compare the sensitivity of bioautography with the disk test for quantitative measurement of gentamicin.
    • To evaluate bioautography as a superior assay method for antibiotic quantification.

    Main Methods:

    • Gentamicin was subjected to chromatographic separation.
    • Quantitative analysis was performed using both bioautography and the disk diffusion test.
    • Inhibition zone sizes produced by known gentamicin concentrations were measured.

    Main Results:

    • Bioautography produced inhibition zones approximately 7 times larger than those from the disk test for equivalent gentamicin amounts.
    • This indicates a higher sensitivity of the bioautography method.
    • The larger zones facilitate more precise quantitative measurements.

    Conclusions:

    • Bioautography is a more sensitive and effective assay method for the quantitative determination of gentamicin concentrations.
    • This enhanced sensitivity can improve antibiotic monitoring and therapeutic drug management.

    Related Experiment Videos