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Related Experiment Videos

Cytoskeleton changes in fibroblast adhesion and detachment.

R A Badley, A Woods, L Carruthers

    Journal of Cell Science
    |June 1, 1980
    PubMed
    Summary

    This study reveals that stress fibers, a component of the cytoskeleton, directly correlate with focal adhesions in fibroblasts. Stress fibers are key targets for agents causing cell detachment.

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    Area of Science:

    • Cell Biology
    • Cytoskeleton Dynamics
    • Cell Adhesion

    Background:

    • Fibroblast organization is influenced by anchorage-dependent growth and spreading.
    • Cytoskeleton components and focal adhesion patterns require detailed comparison.

    Purpose of the Study:

    • To investigate the relationship between cytoskeleton organization and focal adhesion patterns in fibroblasts.
    • To determine which cytoskeleton elements are associated with focal adhesions.

    Main Methods:

    • Immunofluorescence microscopy was used to stain actin, tubulin, and 10-nm filament protein.
    • Interference-reflexion microscopy visualized focal adhesions.
    • Fibroblast spreading on fibronectin-treated glass was analyzed.

    Main Results:

    • Stress fibers, but not actin geodesic domes, microtubules, or 10-nm filaments, directly correlated with focal adhesion patterns.
    • Focal adhesions were located beneath stress fiber termini.
    • During cell rounding induced by EGTA or trypsin, stress fibers dispersed before shape change, while other filaments altered distribution post-change.

    Conclusions:

    • Stress fibers are the primary cytoskeleton feature linked to focal adhesions.
    • Stress fibers are direct targets for agents causing cell rounding and detachment.
    • Differential dispersal mechanisms of stress fibers by EGTA and trypsin were observed.

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