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A simple method for the isolation of phages from Listeria monocytogenes
Zentralblatt Fur Bakteriologie. 1. Abt. Originale. A: Medizinische Mikrobiologie, Infektionskrankheiten Und Parasitologie
|February 1, 1980
Abstract:
By application of a combined mitomycin-/heat treatment after freezing, 7 out of 29 Listeria monocytogenes strains which were found to be non-phage carriers by UV irradiation could release phages. Propagation of the obtained phages was promoted by storage at 4 degrees C. Apart from TNSA plates the application of chocolate plates appears to be necessary in order to study these phages.
Insights
Researchers induced bacteriophage release from Listeria monocytogenes using mitomycin and heat treatment. Storing phages at 4°C enhanced propagation, with chocolate plates proving essential for study.
Area of Science:
- Microbiology
- Bacteriology
- Virology
Background:
- Listeria monocytogenes is a significant foodborne pathogen.
- Bacteriophages can be used for pathogen detection and control.
- Inducing prophage release is crucial for studying bacteriophages.
Purpose of the Study:
- To investigate methods for inducing bacteriophage release from non-lysogenic Listeria monocytogenes strains.
- To optimize conditions for bacteriophage propagation.
- To identify suitable culture media for bacteriophage study.
Main Methods:
- Application of combined mitomycin and heat treatment to frozen Listeria monocytogenes strains.
- UV irradiation was used to initially screen for non-phage carriers.
- Storage of induced phages at 4°C.
- Cultivation on TNSA and chocolate agar plates.
Main Results:
- Phages were successfully induced and released from 7 out of 29 Listeria monocytogenes strains.
- Storage at 4°C significantly promoted the propagation of the released bacteriophages.
- Chocolate agar plates were found to be necessary for effective study of these phages, in addition to TNSA plates.
Conclusions:
- Combined mitomycin and heat treatment is an effective method for inducing bacteriophage release from specific Listeria monocytogenes strains.
- Refrigerated storage enhances bacteriophage propagation.
- Specialized culture media, like chocolate agar, are vital for studying certain bacteriophages.