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Morphometry of human leukocytes.

G W Schmid-Schönbein, Y Y Shih, S Chien

    Blood
    |November 1, 1980
    PubMed
    Summary
    This summary is machine-generated.

    Quantitative models of leukocyte functions require accurate cell measurements. This study reveals white blood cells (leukocytes) are spherical with folded membranes, differing significantly from blood smear measurements.

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    Area of Science:

    • Cell Biology
    • Hematology
    • Biophysics

    Background:

    • Accurate morphometric parameters of white blood cells (leukocytes) are crucial for quantitative functional models.
    • Previous measurements may be affected by preparation artifacts.

    Purpose of the Study:

    • To establish quantitative models of leukocyte functions by determining key morphometric parameters.
    • To assess potential artifacts in transmission electron microscopy (TEM) preparation by comparing with light microscopy (LM).

    Main Methods:

    • Stereologic methods applied to TEM of random human white blood cell sections (neutrophils, lymphocytes, monocytes, eosinophils).
    • Comparative analysis between TEM and LM to evaluate preparation artifacts and cell shrinkage.

    Main Results:

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    • Undeformed white blood cells in isotonic solution are spherical with extensive membrane foldings.
    • Cell diameter measured in suspension is significantly smaller than on blood smears.
    • Chemical fixation minimizes shrinkage to below LM resolution.
    • Leukocytes possess excess membrane area relative to their volume, which is maintained during hypotonic swelling.

    Conclusions:

    • TEM with stereology provides accurate morphometric data for leukocytes.
    • Cell preparation significantly impacts measured dimensions; spherical shape with folded membranes is the native state.
    • Leukocyte membrane area is surprisingly abundant and stable, with implications for cell function and volume regulation.