Influence of aqueous fixation on articular surface morphology. A reflected light interference microscope study.

Area of Science:

• Biomaterials Science
• Orthopedics
• Microscopy

Background:

• The temporomandibular joint (TMJ) is crucial for mastication and speech.
• Understanding the native state of TMJ articular surfaces is vital for research.
• Preservation methods can impact morphological integrity.

Purpose of the Study:

• To evaluate the effects of aqueous fixation on TMJ articular surface morphology.
• To assess the utility of reflected light interference microscopy (RLIM) in detecting fixation-induced changes.

Main Methods:

• Examination of fresh, unfixed baboon TMJ fibrous articular surfaces.
• Application of reflected light interference microscopy (RLIM) for high-resolution imaging.
• Comparison of unfixed surfaces with those fixed in buffered glutaraldehyde and formalin.

Main Results:

• Fixation in buffered glutaraldehyde and formalin induced significant, measurable changes in articular surface morphology.
• RLIM effectively captured and visualized these fixation-induced alterations.
• Unfixed surfaces exhibited distinct morphological characteristics compared to fixed specimens.

Conclusions:

• Aqueous fixation methods demonstrably alter the morphology of TMJ fibrous articular surfaces.
• Reflected light interference microscopy is a sensitive and valuable tool for assessing fixation artifacts in joint tissues.
• Careful consideration of fixation techniques is necessary when studying TMJ articular surface morphology.