Clonal antibodies for core protein of chondroitin sulfate proteoglycan

Antibodies have been prepared to the core protein of cartilage chondroitin sulfate proteoglycan from clones isolated after fusion of rat spleen cells and mouse myeloma cell lines. Antibodies produced by five clones were studied in detail by a radioimmunoassay utilizing 35SO42-labeled hyaluronidase-digested chondroitin sulfate proteoglycan. Preparations of antigen were shown to contain at least two antigenic determinants, one of which was restricted to a portion of antigen in these preparations. One clone was shown to react with proteoglycan synthesized in a cell-free system. It is proposed that such clonal antibodies can be used for structural and biosynthetic studies of core protein.